Technical Data
Z0900-01B
ZW10 Interactor 1 (ZW10-interacting Protein 1, ZWINT 1, ZWINT1, ZWINT-1, ZW10 Interactor, ZWINT, Human ZW10 Interacting Protein 1, HZwint 1, HZwint-1, KNTC2AP, MGC117174)
Description:
ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide between amino acids 66-96 in the center region of human ZWINT
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human ZWINT. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Famulski,J.K., J. Cell Biol. 180(3), 507-520 (2008). 2. Kops,G.J., J. Cell Biol. 169(1), 49-60 (2005). 3. Wang,H., J. Biol. Chem. 279(52), 54590-54598 (2004).