Implicated in endocytosis. May recruit other proteins to membranes with high curvature.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:32,000 Western Blot: 0.3-1ug/ml, observed in lysates of cell line K562 on ~48kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-EPFDLGEPEQSN, from the internal region of human SH3GL1 (NP_003016.1). Species sequence homology: bovine, canine
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SH3GL1.
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