CRISPR Reagents

The CRISPR-Cas9 system is a gene editing technique that can target and modify the genomic DNA of mammalian cells. CRISPR-Cas9 is faster, cheaper and more accurate than previous DNA editing techniques. The enzyme 'Cas-9' acts as a 'molecular knife' that can cut the two strands of DNA at a specific location on the genome. DNA can then be added or removed from that location.

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 (CRISPR associated protein 9) system is the RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. Cas9 protein of the CRISPR-Cas9 system is an RNA-guided DNA endonuclease enzyme that forms a very stable ribonucleoprotein (RNP) complex with the single-guide RNA (sgRNA) and unwinds the genomic DNA duplex. This leads to a disruption in the open reading frame of the targeted gene. United States Biological offers a wide range of CRISPR tools including the highly pure recombinant S. pyogenes and S. aureus Cas9 Proteins, CRISPR Genomic Cleavage Detection Kit, and sgRNA Synthesis Kits for Genome Editing.

Applications:

• RNA-guided Genome Editing
• CRISPR Genomic Cleavage Detection Kit to Verify the Genomic Editing Process
• Simple CRISPR sgRNA Synthesis Kit for the Production of sgRNA

Key Features:

• Ready-To-Use, Highly Pure and Efficient S. pyogenes and S. aureus Cas9 Proteins
• Wide Range of Cas9 Proteins (Cas9 Nucleases, Nickases, Double Mutants) to Choose From
• Injection and Transfection Ready Cas9 Proteins
• GFP-Cas9 Proteins for Genomic Locus Visualization
• Ready-to-Use, Easy and Effective CRISPR Genomic Cleavage Detection Kit to Verify Genomic Editing Process
• Simple and Robust Production of sgRNA using CRISPR sgRNA Synthesis Kits

For a more complete and detail product listing, click below:

• Molecular Biology

For a more complete and detail product listing, click below:

• Biochemicals