A Broth is a minimal medium base used in the maintenance and propagation of E.coli.
Dissolve 16.5 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Component | g/liter |
|---|---|
| Ammonium Sulfate | 1 |
| Potassium Phosphate Dibasic | 10.5 |
| Potassium Phosphate Mono | 4.5 |
| Sodium Citrate | 0.5 |
| Total: 16.5g/Liter | |
| 500g makes 32 liters |
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