The activity of an antibiotic can be demonstrated under suitable conditions by its inhibitory effect on organisms. Antibiotic assays are performed by the cylinder plate method and the turbidimetric assay. These media has been formulated according to the specifications and procedures listed by the Food and Drug Administration, European Pharmacopeia, Code of Federal Regulations, and the USP. The Antibiotic Media are identified numerically and also, where applicable, with names assigned by Grove and Randall in Assay Methods of Antibiotics. Antibiotic Medium #11 is recommended for determing antibotic potency to neymycin.
Dissolve 43.5grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Pancreatic Digest of Casein | 17.0 |
| Pancreatic Digest of Gelatin | 6.0 |
| Beef Extract | 1.5 |
| Yeast Extract | 3.0 |
| Dextrose | 1.0 |
| Agar | 15.0 |
| Total: 52g/Liter |
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