Sodium azide inhibits Gram negative and Gram positive bacteria, other than enterococci, are inhibited by the bile salts.
Dissolve 56grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Pancreatic Digest of Casein | 17.0 |
| Peptic Digest of Animal Tissue | 3.0 |
| Yeast Extract | 5.0 |
| Oxgall, dehydrated | 10.0 |
| Sodium Chloride | 5.0 |
| Sodium Citrate | 1.0 |
| Esculin | 1.0 |
| Ferric Ammonium (HI) Citrate | 0.5 |
| Sodium Azide | 0.25 |
| Agar | 13.5 |
| Total: 56g/Liter |
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