Selective medium for isolation of Salmonella.
Dissolve 53grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Casein Digest Peptone | 5.0 |
| Sodium Chloride | 5.0 |
| Peptic Digest of Animal | 5.0 |
| Beef Extract | 5.0 |
| Disodium Phosphate | 5.0 |
| Ferrous Sulfate | 0.3 |
| Bismuth Sulfite Indicator | 8.0 |
| Brilliant Green | 0.025 |
| Agar | 20.0 |
| Total: 53g/Liter |
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