Highly nutrituve medium, when supplemented: an isolation medium for Campylobacter sp.
Dissolve 42grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi). Prepare 5 to 10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium. Cool to 45-50°C. Dispense into Petri dishes or tubes.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Casein Digest Peptone | 7.5 |
| Liver Digest | 5.0 |
| Peptic Digest of Animal Tissue | 7.5 |
| Sodium Chloride | 5.0 |
| Yeast Extract | 5.0 |
| Agar | 12.0 |
| Total: 42g/Liter |
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