Brucella Agar is a prepared medium for the cultivation of Brucella sp. from clinical and dairy products. With the addition of blood and menadione, Brucella Agar can be used to cultivate anaerobic bacteria. Cultivation of Brucella sp. requires CO2 enriched
Suspend 43 grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). To prepare blood plates, cool medium to 45°- 50.0°C and add aseptically 5% sheep red blood or 10% horse blood. Dispense into sterile Petri plates.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Component | g/liter |
|---|---|
| Casein Digest Peptone | 10.0 |
| Yeast Extract | 2.0 |
| Sodium Chloride | 5.0 |
| Sodium Bisulfite | 0.1 |
| Peptic Digest of Animal Tissue | 10.0 |
| Dextrose | 1.0 |
| Agar | 15.0 |
| Total: 43g/Liter |
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