Addition of acid fuchsin produces pink colonies by lactose fermenters.
CLED Agar (Cystine-Lactose-Electrolyte-Deficient) media was first formulated by Sandys and later modified by Mackey and Sand. In the presence of the indicator dye, bromthymol blue, lactose-fermenting bacteria produce yellow colonies while non-lactose fermenting bacteria give blue colonies. Bevis incorporated an additional dye, acid fuchsin, causing lactose fermenting bacteria to produce bright pink colonies and non-lactose fermenting bacteria to show colorless. This medium supports the growth of urinary pathogens and give good colonial differentiation of these bacteria. Since there are no salts (electrolyte-deficient) in this media the swarming of Proteus sp. is suppressed. Various authors have used CLED Agar, Bevis as a dip inoculum transport medium for urine specimens. A quantitative count method for urine cultures called the Drop Plate method was devised by Neblett.
Dissolve 36.2grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Pancreatic Digest of Gelatin | 4.0 |
| Beef Extract | 3.0 |
| Casein Digest Peptone | 4.0 |
| Lactose | 10.0 |
| L-Cystine | 0.128 |
| Bromothymol Blue | 0.02 |
| Acid Fuchsin | 0.1 |
| Agar | 15.0 |
| Total: 36.2g/Liter |
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