D/E Neutralizing Agar is capable of neutralizing a broad spectrum of antiseptic and disinfectant chemicals including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde and glutaraldehyde. It can determine the bactericidal capability of disinfectants and therefore is well suited for environmental sampling.
Dissolve 54g/liter in ddH2O with heat. stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Yeast Extract | 2.5 |
| Dextrose | 10.0 |
| Casein Digest Peptone | 5.0 |
| Lecithin | 7.0 |
| Sodium Thioglycollate | 1.0 |
| Sodium Thiosulfate | 6.0 |
| Sodium Bisulfite | 2.5 |
| Polysorbate 80 | 5.0 |
| Bromocresol Purple | 0.02 |
| Agar | 15.0 |
| Total: 54g/Liter |
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