Dextrose Broth is a basic medium used to study the fermentation of dextrose by microorganisms. It contains casein peptone used as carbon and nitrogen source and salt for electrolyte balance. Dextrose is the only fermentable carbohydrate. The medium does not contain a pH indicator.
Dissolve 20grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Casein Digest of Peptone | 10.0 |
| Dextrose | 5.0 |
| Sodium Chloride | 5.0 |
| Total: 20g/Liter |
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