Media for propagation of bacteriophage lambda.
Dissolve 35.98 grams per liter of distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| NZ Amine | 10 |
| NaCl | 10 |
| MgCl2 | 0.98 |
| Yeast Extract | 5 |
| Agar | 15 |
| Total: 35.98g/Liter | |
| 500g makes 13.9 liters |
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