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Papanicolaou's Stain


Recipe

  1. Mix 11.25ml of a 10% eosin Y solution into 450ml of 100% ethanol.
  2. Mix 2.25ml of a 10% aqueous solution of light green SF into the solution from step 1.
  3. Mix 25ml of a 1% bismarck brown solution (in ethanol) into the solution from step 2.
  4. Mix 10ml of a 10% aqueous solution of phosphotongstic acid into the solution from step 3.
  5. Mix 0.3ml of an aqueous solution of saturated lithium carbonate into the solution from step 4.
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