Dissolve 32g in 800-900ml of ddH2O stirring gently until completely solubilized. If required add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Inorganic Salts | mg/liter |
|---|---|
| Calcium Chloride•2H2O | 8.20 |
| Ferric Chloride•6H2O | 2.69 |
| Magnesium Chloride | 95.35 |
| Manganese Sulfate•4H2O | 0.36 |
| Potassium Phosphate Dibasic | 381.70 |
| Zinc Sulfate•7H2O | 0.80 |
| Amino Acids | |
| L-Arginine HCl | 1270.00 |
| L-Asparagine•H2O | 1136.00 |
| L-Cysteine•HCl•H2O | 1450.00 |
| L-Cystine | 221.70 |
| L-Glutamine | 2520.00 |
| Glycine | 500.00 |
| L-Histidine•HCl•H2O | 419.00 |
| L-Isoleucine | 520.00 |
| L-Leucine | 520.00 |
| L-Lysine•HCI | 724.65 |
| L-Methionine | 150.00 |
| L-Phenylalanine | 320.00 |
| L-Proline | 1000.00 |
| L-Threonine | 480.00 |
| L-Tryptophan | 100.00 |
| L-Tyrosine•Na2•2H2O | 519.00 |
| L-Valine | 460.00 |
| Vitamins | |
| Biotin | 0.50 |
| Choline Chloride | 25.00 |
| Folic Acid | 5.00 |
| i-Inositol | 25.00 |
| Niacinamide | 5.00 |
| D-Pantothenic Acid•Ca | 25.00 |
| Pyridoxal•HCI | 5.00 |
| Riboflavin | 5.00 |
| Thiamine•HCI | 5.00 |
| Other | |
| Adenine | 25.00 |
| Ammonium Acetate | 500.00 |
| Fumaric Acid | 1500.00 |
| D-Glucose | 1000.00 |
| MOPS•Na (65mM) | 15030.00 |
| Phenol Red, Sodium | 2.00 |
| Sodium Pyruvate | 1000.00 |
| Uracil | 25.00 |
| Total: 31.98g/Liter |
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