SAAMF (Synthetic Amino Acid Medium-Fungal) is a completely defined, highly buffered medium developed to support the growth of a wide spectrum of fungi. SAAMF has been demonstrated to be nonantagonistic to any presently known antifungal agent and is preferred to media containing naturally derived components.
Dissolve 50.93g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Inorganic Salts | mg/liter |
|---|---|
| Calcium Chloride•2H2O | 8.20 |
| Ferric Chloride•6H2O | 2.69 |
| Magnesium Chloride | 95.35 |
| Manganese Sulfate•4H2O | 0.36 |
| Potassium Phosphate Dibasic | 381.70 |
| Zinc Sulfate•7H2O | 0.80 |
| Amino Acids | |
| L-Arginine HCl | 1270.00 |
| L-Asparagine•H2O | 1136.00 |
| L-Cysteine•HCl•H2O | 1450.00 |
| L-Cystine | 221.70 |
| L-Glutamine | 2520.00 |
| Glycine | 500.00 |
| L-Histidine•HCl•H2O | 419.00 |
| L-Isoleucine | 520.00 |
| L-Leucine | 520.00 |
| L-Lysine•HCI | 724.65 |
| L-Methionine | 150.00 |
| L-Phenylalanine | 320.00 |
| L-Proline | 1000.00 |
| L-Threonine | 480.00 |
| L-Tryptophan | 100.00 |
| L-Tyrosine•Na2•2H2O | 519.00 |
| L-Valine | 460.00 |
| Vitamins | |
| Biotin | 0.50 |
| Choline Chloride | 25.00 |
| Folic Acid | 5.00 |
| i-Inositol | 25.00 |
| Niacinamide | 5.00 |
| D-Pantothenic Acid•Ca | 25.00 |
| Pyridoxal•HCI | 5.00 |
| Riboflavin | 5.00 |
| Thiamine•HCI | 5.00 |
| Other | |
| Ammonium Acetate | 500.00 |
| Fumaric Acid | 1500.00 |
| D-Glucose | 20000.00 |
| MOPS•Na (65mM) | 15030.00 |
| Phenol Red, Sodium | 2.00 |
| Sodium Pyruvate | 1000.00 |
| Total: 50.93g/Liter |
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