Terrific Broth is a highly enriched bacteriological medium specifically designed by Tartof and Hobbs to increase the yield of plasmid DNA derived from suspension cultures of E. coli due in part to the increased buffering capacity derived from using mono and dibasic phosphates. T2810-05 is specially formulated with pyruvic acid as an alternate carbon source, eliminating the need to add glycerol. Provides the same high yield of plasmid DNA as traditional Terrific Broth.
Dissolve 51.6 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). If precipitate forms after autoclaving, medium may be filter-sterilized using a 0.2 micron filter.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Potassium phosphate dibasic | 9.4 |
| Potassium phosphate monobasic | 2.2 |
| Sodium Pyruvate | 4 |
| Tryptone | 12 |
| Yeast Extract | 24 |
| Total: 51.6g/Liter | |
| 500g makes 9.7 liters |
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