Castenholz TYE media is designed to support the growth of Thermophilus as it is too nutrient-poor to support many other bacterial life.
Dissolve 4.052g per liter of ddH2O, stirring gently until completely solubilized. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Note: The 100X liquid medium forms precipitates at pH values greater than 5. If higher pH is required, it is advisable to prepare a 2X solution from the stock 100X solution, and adjust this solution to the desired pH. Filter-sterilize using a 0.22 micron membrane filter. Adjust the volume accordingly for use with Thermophilus Growh Medium Base Broth.
Store powdered media at 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Minerals | g/liter |
|---|---|
| Boric Acid | 0.05 |
| Calcium Chloride | 0.66 |
| Cobalt Nitrate | 0.005 |
| Copper Sulfate | 0.015 |
| Ferric Chloride | 0.50 |
| Magnesium Chloride | 2.50 |
| Manganese Sulfate | 0.20 |
| Nickel Chloride | 0.002 |
| Sodium Molybdate | 0.0025 |
| Zinc Sulfate | 0.05 |
| Vitamins | |
| p-Aminobenzoate | 0.008 |
| Biotin | 0.001 |
| Choline Bromide | 0.005 |
| Cyanocobalamin | 0.00004 |
| Folic Acid | 0.001 |
| myo-Inositol | 0.002 |
| Nicotinamide | 0.008 |
| Monosodium Orotic Acid | 0.002 |
| D-Pantothenic Acid•Ca | 0.008 |
| Pyridoxine Hydrochloride | 0.008 |
| Riboflavin | 0.004 |
| Spermidine | 0.010 |
| Thiamine Hydrochloride | 0.010 |
| Total: 4.052g/Liter |
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