Castenholz TYE media is designed to support the growth of Thermophilus as it is too nutrient-poor to support many other bacterial life.
Dissolve 40.51g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Minerals | g/liter |
|---|---|
| Boric Acid | 0.50 |
| Calcium Chloride | 6.60 |
| Cobalt Nitrate | 0.05 |
| Copper Sulfate | 0.15 |
| Ferric Chloride | 5.00 |
| Magnesium Chloride | 25.00 |
| Manganese Sulfate | 2.00 |
| Nickel Chloride | 0.02 |
| Sodium Molybdate | 0.025 |
| Zinc Sulfate | 0.50 |
| Vitamins | |
| p-Aminobenzoate | 0.08 |
| Biotin | 0.01 |
| Choline Bromide | 0.05 |
| Cyanocobalamin | 0.0004 |
| Folic Acid | 0.01 |
| myo-Inositol | 0.02 |
| Nicotinamide | 0.08 |
| Monosodium Orotic Acid | 0.02 |
| D-Pantothenic Acid•Ca | 0.08 |
| Pyridoxine Hydrochloride | 0.08 |
| Riboflavin | 0.04 |
| Spermidine | 0.10 |
| Thiamine Hydrochloride | 0.10 |
| Total: 40.515g/Liter |
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