HSMT3, Sentrin 2, Small Ubiquitin like Modifier 2, Small Ubiquitin like Modifier Protein 3, SMT3A, SMT3B, SMT3 Homolog 1, SMT3H1, SMT3 Homolog 2, SMT3H2, SMT3 Homolog, SMT3 Suppressor of mif two 3 Homolog 1, SMT3 Suppressor of mif two 3 Homolog 2, SMT3 Suppressor of mif two 3 Homolog 3, Suppressor of mif two 3 Homolog 2, Suppressor of mif two 3 Homolog 3, Ubiquitin like Protein SMT3A, Ubiquitin like Protein SMT3B
This MAb reacts with both SUMO-2 and SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer’s disease.
Applications
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1ug/ml Western Blot: 0.5-1ug/ml Immunoprecipitation: 0.5-1µg/500ug protein lysate Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
Positive Control
HeLa cells or breast carcinoma
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant human SUMO2 protein. Cellular Localization: Predominantly nuclear with some cytoplasmic
Form
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human SUMO-2/3. Shows broad species reactivity.