AP2A1, Adaptin, alpha A, ADTAA, Alpha-adaptin A, Alpha1-adaptin, AP-2 complex subunit alpha-1, AP2 Alpha, CLAPA1, AP2-alpha
This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:128,000 Western Blot: 0.01-0.03ug/ml Immunohistochemistry(Paraffin): 5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-ELEPPAPESPMALLADPAPAAD from an internal region of human AP2A1 (NP_055018.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Ferret, Canine, Feline, Panda, Equine, Rabbit, Porcine (100%); Rat (95%); Opossum, Guinea Porcine (91%).
Form
Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human AP2A1. Expected to recognize reported isoform 1 (NP_055018.2) only. Species Crossreactivity: Gibbon, Chimpanzee, Canine, Gorilla, Equine, Monkey, Mouse, Porcine, Rabbit.
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