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G2033-91 Rabbit Anti-Gi alpha 3 (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P08754
Isotype
IgG
Grade
Serum
Applications
IP WB
Crossreactivity
Hm Hu Mo Rt
Accession #
NM_006496
Shipping Temp
Blue Ice
Storage Temp
-20°C

Gi alpha 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-gamma activation and Ca+2 mobilization in hepatocytes1,2. G-proteins are membrane associated heterotrimeric proteins that are comprised of alpha, beta-, and lambda-subunits. The alpha-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the alpha-subunit from the beta, lambda-subunit complex. This enables the G alpha-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the alpha-subunit with the beta, lambda- subunit complex.

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000-1:2000 detected Gi alpha 3 in rat hippocampus membrane preparation. Previous lots detected Gi apha 3 in solubilized rat and mouse brain membranes and mouse 3T3/A31 cell lysates. Immunoprecipitation: 4ul immunoprecipitated Gi alpha 3 from a solubilized mouse brain membrane lysate. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 10aa, KNNLKECGLY from the C-terminal region of Gi alpha 3.
Form
Supplied as a liquid in PBS, pH 7.4.
Purity
Serum
Specificity
Recognizes Gi alpha 3 and Go alpha, Mr 40kD. Low crossreactivity with other G-proteins. Species Crossreactivity: human, mouse, hamster and rat
References
1. Lynch, C J, et al (1989). J Clin Invest 83: 2050-62. 2. Barrett, P., et al (2000). A role for tyrosine phosphorylation in the regulation and sensitization of adenylate cyclase by melatonin. Faseb J 14: 1619-28. 3. Ward, D. T., et al (1998). Disulfide bonds in the extracellular calcium-polyvalent cation-sensing receptor correlate with dimer formation and its response to divalent cations in vitro. J Biol Chem 273: 14476-83. 4. Sands, J. M., et al (1997). Apical extracellular calcium/polyvalent cation-sensing receptor regulates vasopressin-elicited water permeability in rat kidney inner medullary collecting duct. J Clin Invest 99: 1399-405. 5. van Biesen, T., et al (1996). G(o)-protein alpha-subunits activate mitogen-activated protein kinase via a novel protein kinase C-dependent mechanism. J Biol Chem 271: 1266-9.
USBio References
No references available
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