Podoplanin is absent on blood endothelium and therefore this product is readily able to distinguish between lymphatic vessels and blood vasculature. Although the specific function of this protein is undetermined, Podoplanin is reported to be a reliable indicator of tumor lymphangiogenesis.

Suitable for use in Immunohistochemistry. Other applications not tested.

Immunohistochemistry (Frozen and paraffin): 0.75-2ug/ml Note: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Use sodium citrate buffer, pH 6.0. Optimal dilution determined by the researcher.

I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo

I1904-78R: IgG1 Murine Negative Control

Normal lung

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

ORF Human podoplanin ectodomaine expressed in E. coli.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes Human Podoplanin (gp36), a 36kD type I cell membrane-associated glycoprotein, with a broad spectrum of distribution, being strongly expressed on the lymphatic endothelium found in lung, placenta and skeletal muscle and to a lesser extent in brain, kidney and liver.