Myc gene was first discovered in Burkitt's lymphoma patients. In Burkitt's lymphoma, cancer cells show chromosomal translocations, in which Chromosome 8 is frequently involved. Cloning the break point of the fusion chromosomes revealed a gene that was similar to myelocytomatosis viral oncogene (v-Myc). The new cellular gene was named c-Myc. Human c-myc (accession # P01106; 439aa, chromosome 8q24) is a protooncogene, which is overexpressed in a wide range of human cancers. When it is specifically-mutated, or overexpressed, it increases cell proliferation and functions as an oncogene. It is a transcription factor that regulates expression of a great number of genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). Myc belongs to Myc family of transcription factors, which also includes N-Myc and L- Myc genes. Myc-family transcription factors contain the bHLH/LZ (basic Helix-Loop-Helix Leucine Zipper) domain.

An epitope located within aa410-419 (EQKLISEEDL) of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tag fusion proteins. Epitope tags antibodies provide a method to immunolocalize the myc-fusion gene products in a variety of cell types, to study the topology of proteins and protein complexes, and to identify associated proteins. In addition, they allow characterization of newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. It is also possible to use ant-tag antibodies for the purification of fusion proteins. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.

Recombinant protein corresponding to full-length human C-myc, expressed as fusion protein (His-tag-Myc), in E. coli.

~65kD

Suitable for use as Positive Control for Western Blot. Not suitable for ELISA or other applications where native protein is required. Other applications not tested.

Western Blot: 1-2ug/ml using Chemiluminescence technique Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

E. coli

Highly Purified (~95%)

Not determined

Supplied as a powder in a SDS-PAGE sample buffer (reduced). Reconstitute with 100ul of 1X sample buffer (reduced). If stored for several weeks, add 5ul of fresh 2x sample buffer (reducing) /10ul of the solution prior to heating and loading on gels.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.