NFAT (Nuclear Factor of activated T Cells) is composed by five members: NFATC (1, 2, 3 and 4) and NFAT5. Genes encoding NFAT proteins are transcribed in almost every tissue, although the expression of individual proteins is more tissue restricted. When intracellular calcium rises, the calmodulin-dependent serine-threonine phosphatase calcineurin dephosphorylates NFAT proteins in the cytoplasm, exposing their NLS (Nuclear Localization Sequence) and inducing their translocation to the nucleus. NFATc4 protein is expressed at highest levels in the developing hippocampus at about the time of birth and responds to normal synaptic activity, which transduces Ca2+ signals mediated by NMDA receptors and L-type Ca2+ channels.

Suitable for use in ELISA. Other applications not tested.

Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Purified

As reported

Supplied as a lyophilized powder.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.