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029119 Rabbit Anti-DAD1 (Dolichyl-diphosphooligosaccharide-protein Glycosyltransferase Subunit DAD1, Oligosaccharyl Transferase Subunit DAD1, Defender Against Cell Death 1, DAD-1) (HRP)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P61803
Conjugate
HRP
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Immunogen
Recombinant protein corresponding to aa1-113 from human DAD1.
Form
Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human DAD1.
References
1. "Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells."Nakashima T., Sekiguchi T., Kuraoka A., Fukushima K., Shibata Y., Komiyama S., Nishimoto T.Mol. Cell. Biol. 13:6367-6374 (1993). 2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.Submitted (JUN-2004) 3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127 (2004).
USBio References
No references available
Conjugates
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