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030138 Mouse Anti-Cytokeratin(Pan)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P13647
Isotype
IgG1
Clone Number
13B216
Grade
Ascites
Applications
E IF IHC
Crossreactivity
Hu
Gene ID
3852
Shipping Temp
Blue Ice
Storage Temp
-20°C
K5; DDD; EBS2; KRT5A; KRT5

Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors.

Applications
Immunohistochemistry: 1:200-1:1000. Immunofluorescence: 1:200-1:1000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of Cytokeratin 5 expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Purity
Ascites
Specificity
Human
References
1. Vet Rec. 2006, Dec 16, 159(25): 839-43. |2. J Cell Biochem. 2007, Apr 15, 100(6): 1406-14.
USBio References
No references available
Conjugates
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