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031733 Rabbit Anti-AKAP4, NT (AKAP4, AKAP82, A-kinase anchor protein 4, A-kinase anchor protein 82kD, Major sperm fibrous sheath protein, Protein kinase A-anchoring protein 4)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q5JQC9
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_003877.2; NP_647450.1
Gene ID
8852
Gene #
AKAP4
Shipping Temp
Blue Ice
Storage Temp
-20°C
AKAP4, AKAP82, A-kinase anchor protein 4, A-kinase anchor protein 82kD, Major sperm fibrous sheath protein, Protein kinase A-anchoring protein 4

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 10-36 in the N-terminal region of human AKAP4, UniProt Accession #Q5JQC9.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human AKAP4.
References
Moretti, E., et al. Asian J. Androl. 9(5):641-649(2007)Lefievre, L., et al. Proteomics 7(17):3066-3084(2007) Ross, M.T., et al. Nature 434(7031):325-337(2005) Brown, P.R., et al. Biol. Reprod. 68(6):2241-2248(2003) Ficarro, S., et al. J. Biol. Chem. 278(13):11579-11589(2003)
USBio References
No references available
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