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032233 Rabbit Anti-ATG7 (APG7-LIKE, APG7L, DKFZp434N0735, GSA7, APG7 autophagy 7-like, autophagy-related protein 7, hAGP7, ubiquitin activating enzyme E1-like protein, ubiquitin-activating enzyme E1-like protein)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
O95352
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC
Crossreactivity
Hu
Gene ID
10533
Gene #
ATG7
Shipping Temp
Blue Ice
Storage Temp
-20°C
APG7-LIKE, APG7L, DKFZp434N0735, GSA7, APG7 autophagy 7-like, autophagy-related protein 7, hAGP7, ubiquitin activating enzyme E1-like protein, ubiquitin-activating enzyme E1-like protein

APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

Applications
Suitable for use in Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80~110 amino acids surrounding S95 of human APG7L.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human
References
Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) |Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) |Greenberg JT. Dev Cell. 8(6):799-801. (2005) |Levine B. Cell. 120(2):159-62. (2005) |Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)|Tanida I., et al. Biochem. Biophys. Res. Commun. 292:256-262(2002)| Tanida I., et al. J. Biol. Chem. 277:13739-13744(2002)
USBio References
No references available
Conjugates
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