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038607 Rabbit Anti-MRPS15, ID (MRPS15, RPMS15, 28S ribosomal protein S15, mitochondrial)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P82914
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_112570.2
Gene ID
64960
Gene #
MRPS15
Shipping Temp
Blue Ice
Storage Temp
-20°C
MRPS15, RPMS15, 28S ribosomal protein S15, mitochondrial

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MRPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-153 amino acids from the Central region of human MRPS15.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human
References
Zhang, Z., et al. Genomics 81(5):468-480(2003)|Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :|Suzuki, T., et al. J. Biol. Chem. 276(35):33181-33195(2001)|Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001)
USBio References
No references available
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