Prolactin acts primarily on the mammary gland by promoting lactation.
Applications
Suitable for use in Indirect Flow Cytometry, ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Indirect Flow Cytometry: 1:10-1:50 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 188-216 in the C-terminal region of human PRL, UniProt Accession #NP_000939.1; NP_001157030.1; P01236. Species sequence homology: porcine, rabbit
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human PRL.
References
1. Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010) 2. Strauss, J.S., et al. Psychoneuroendocrinology 35(9):1422-1428(2010) 3. Gordon, I., et al. Horm Behav 58(3):513-518(2010) 4. Voorhees, J.L., et al. J. Biol. Chem. 285(26):20022-20030(2010) 5. Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010)