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042697-APC Rabbit Anti-TCF3, CT (TCF3, BHLHB21, E2A, ITF1, Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, Transcription factor ITF-1) (APC)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P15923
Conjugate
APC
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IHC WB
Crossreactivity
Hu Mo
Accession #
NP_003191.1; NP_001129611.1
Gene ID
6929
Gene #
TCF3
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
TCF3, BHLHB21, E2A, ITF1, Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, Transcription factor ITF-1

The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).

Applications
Suitable for use in Western Blot, Immunohistochemistry and FLISA. Other potential application, though not tested is Direct Flow Cytometry. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 506-534 in the C-terminal region of human TCF3.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human TCF3. Species Crossreactivity: mouse.
References
1. Hirose, K., et al. Blood 116(6):962-970(2010) 2. Pan, F., et al. Immunogenetics 62(4):237-251(2010) 3. Hauser, J., et al. Mol. Immunol. 47(5):1031-1038(2010) 4. Rittie, L., et al. Aging Cell 8(6):738-751(2009) 5. Pedraza, N., et al. J. Biol. Chem. 284(47):32980-32988(2009)
USBio References
No references available
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