Angiopoietin-like Protein 3, Angiopoietin-5, ANG-5
Seven proteins containing an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, both characteristics of angiopoietins, have been identified and therefore designated angiopoietin-like proteins 1-7 (ANGPTL 1-7). ANGPTL3 and ANGPTL6 (Angiopoietin-related growth factor; AGF), among others, function to regulate angiogenesis and also appear to directly regulate lipid, glucose, and energy metabolism. ANGPTL3 plasma levels are increased in a mouse model of diabetes mellitus.
The ANGPTL3, mouse/rat, BioAssay™ Dual ELISA Kit is to be used for the in vitro quantitative determination of mouse or rat ANGPTL3 in serum, plasma and cell culture supernatant.
Specificity
Recognizes mouse and rat ANGPTL3. Does not cross-react with human ANGPTL3, mouse ANGPTL4 or human ANGPTL4.
Test Principle
This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse or rat ANGPTL3 in biological fluids. A polyclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a biotinylated polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples.
Kit Components
Plate coated with mouse ANGPTL3 antibody, 1x96wells Wash Buffer 10X, 1x50ml Diluent 5X, 1x50ml Detection Antibody, 1x12ml Detector 100X (HRP Labeled Streptavidin), 1x150ul Mouse ANGPTL3 Standard, lyophilized, 1x2ng Mouse ANGPTL3 QC sample, lyophilized, 1x vial TMB Substrate Solution, 1x12ml Stop Solution, 1x12ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.