Angiopoietin-like Protein 7, Cornea-derived Transcript 6 Protein
ANGPTL7 is expressed in various tissues and regulates angiogenesis. Human ANGPTL7 was characterized as a potent target gene of the WNT/ beta-catenin signaling pathway, and is a pharmacogenomics target in the fields of oncology and regenerative medicine.
Applications
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA (direct or indirect): 1:2000-1:5000 Immunohistochemistry (paraffin sections): 1:100 Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human full length ANGPTL7.
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human ANGPTL7. Detects a band of ~45kD by Western blot. Weakly cross-reacts with human ANGPTL7-CCD.
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