Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, CD40L Receptor
CD40 belongs to the TNF-receptor superfamily and is essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development and germinal center formation. The interaction of CD40-CD40L is necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.
Recombinant protein corresponding to human CD40 (aa 21-193), fused to the Fc portion of human IgG1 at the C-terminus, expressed in HEK293 cells.
Molecular Weight
~54kD (SDS-PAGE)
Specificity
Binds human and mouse CD40L.
Biological Activity
Blocks the binding of CD40L to CD40.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, HEK293 cells
Concentration
~1mg/ml (after reconstitution)
Form
Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Reconstitute with 50ul sterile ddH2O. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Reconstitute with 50ul sterile ddH2O. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Purity
≥95% (SDS-PAGE). Endotoxin: <0.01EU/ug (LAL test; Lonza).
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