ACRP30headless:CD40L, ACRP30headless:CD154, ACRP30headless:TNFSF5, ADIPOQ-CD40L
CD40L is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to combinations. CD40L has shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.
Human CD40L (aa116-261) is fused at the N-terminus to mouse ACRP30headless (aa18-111) and a FLAG®-tag, expressed in CHO cells.
Molecular Weight
~35-40kD (SDS-PAGE)
Biological Activity
Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml).
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, CHO cells
Concentration
~0.1mg/ml (after reconstitution)
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Purity
≥95% (SDS-PAGE). Endotoxin: <0.01EU/ug (LAL test; Lonza).
TDS
NCBI
UniProt
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