IL-23p19 associates with IL-12b to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 binds to an heterodimeric receptor complex composed of IL-12RB1 and IL-23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA (direct or indirect): 1:2000-1:10,000 Western Blot: 1:2000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature. Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human IL-23p19.
Form
Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human IL-23p19.