Nampt, Recombinant, Human

Swiss Prot

P43490

Grade

Highly Purified

Shipping Temp

Blue Ice

Storage Temp

4°C/-20°C

Nicotinamide Phosphoribosyltransferase, Pre-B Cell Colony Enhancing Factor 1, PBEF1

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kD adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Biological Activity

Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.

Sequence

Human Nampt (visfatin/PBEF) (aa1-491) is fused at the N-terminus to a FLAG®-tag.

Molecular Weight

~57kD (SDS-PAGE); ~100kD (Dimer) (SEC)

Storage and Stability

Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source

Recombinant, HEK293 cells

Concentration

~0.1mg/ml (after reconstitution)

Form

Supplied as a lyophilized powder from a 0.2um-filtered solution in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a lyophilized powder from a 0.2um-filtered solution in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

Purity

≥95% (SDS-PAGE). Endotoxin: <0.01EU/ug (LAL test; Lonza).

References

Product Reference: |●Visfatin induces human endothelial VEGF and MMP-2/9 production via MAPK and PI3K/Akt signalling pathways: novel insights into visfatin-induced angiogenesis: R. Adya, et al.; Cardiovasc. Res. 78, 356 (2008) |●ssDNA Aptamer-base-surface plasmon resonance biosensor for the detection of retinol binding protein 4 for the early diagnosis of type 2 diabetes: S.J. Lee, et al.; Anal. Chem. 80, 2867 (2008) |●Pre-B cell colony enhancing factor (PBEF)/visfatin induces secretion of MCP-1 in human endothelial cells: Role in visfatin-induced angiogenesis: R. Adya, et al.; Atherosclerosis 205, 113 (2009) |●Visfatin an Adipocytokine with Proinflammatory and Immunomodulating Properties: A.R. Moschen, et al.; J. Immunol. 178, 1748 (2007) |●Visfatin activates eNOS via Akt and MAP kinases and improves endothelial cell function and angiogenesis in vitro and in vivo: translational implications for atherosclerosis: F. Lovren, et al.; Am. J. Physiol. Endocrinol. Metab. 296, E1440 (2009)|●