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045071 Anti-Nampt, Recombinant, Mouse, His-Tag (Visfatin/PBEF, PBEF1, Pre-B Cell Colony Enhancing Factor 1, Nicotinamide Phosphoribosyltransferase)

Specifications
References
Swiss Prot
Q99KQ4
Grade
Purified
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C
Pre-B Cell Colony Enhancing Factor 1, PBEF1, Nicotinamide Phosphoribosyltransferase

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Biological Activity
Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.
Sequence
Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.
Storage and Stability
Short-term Storage: +4°C Long-term Storage: -20°C Working aliquots are stable for up to 3 months when stored at -20°C.
Source
Recombinant, E. coli
Form
Supplied as a lyophilized powder from a 0.2um-filtered solution in PBS, pH 7.2. Reconstitute in ddH2O.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a lyophilized powder from a 0.2um-filtered solution in PBS, pH 7.2. Reconstitute in ddH2O.
Purity
≥90% (SDS-PAGE). Endotoxin: <1EU/ug (LAL test; Lonza).
References
Product Reference: |●Visfatin regulates insulin secretion, insulin receptor signalling and mRNA expression of diabetes-related genes in mouse pancreatic beta-cells: J.E. Brown, et al.; J. Mol. Endocrinol. 44, 171 (2010)|●
USBio References
No references available
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