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129706-PE Mouse Anti-MKNK1 (MAP Kinase-interacting Serine/Threonine-protein Kinase 1, MAP Kinase Signal-integrating Kinase 1, MAPK Signal-integrating Kinase 1) (PE)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
PE
Isotype
IgG1,k
Clone Number
3E1
Grade
Affinity Purified
Applications
FLISA IF IHC WB
Crossreactivity
Hu
Accession #
BC002755, AAH02755.1
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MNK1 is an MNK protein kinase that is activated in response to mitogens/insulin by ERK1 and ERK2, or in response to stress by activated p38. MNK1 phosphorylates eIF4E to enhance the translation rates of cap-containing mRNAs.

Applications
Suitable for use in Immunohistochemistry, Immunofluorescence, FLISA and Western Blot. Other applications not tested.
Recommended Dilution
Immunofluorescence: 10ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml Optimal dilutions to be determined by the researcher.
AA Sequence
MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTT
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-466 from MKNK1 (AAH02755) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human MKNK1.
Conjugates
Pricing
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