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134300-ML650 Mouse Anti-TCIRG1 (T-cell Immune Regulator 1, ATP6N1C, ATP6V0A3, V-type Proton ATPase 116kD Subunit a Isoform 3, V-ATPase 116kD Isoform a3, Osteoclastic Proton Pump 116kD Subunit, OC-116kD, OC116, T-cell Immune Response cDNA7 Protein, TIRC7, Vacuolar Proton Translocating ATPase 116kD Subunit a Isoform 3) (MaxLight 650)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™650
Isotype
IgG2a,k
Clone Number
6H3
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu
Accession #
BC018133, AAH18133
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
a3, Atp6i, OPTB1, Stv1, Vph1

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Applications
Suitable for use in Western Blot and FLISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
QLHQLQLHAAVLRQGHEPQLAAAHTDGASERTPLLQAPGGPHQDLRVNFVAGAVEPHKAPALERLLWRACRGFLIASFRELEQPLEHPVTGEPATWMTFL
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa121-220 from TCIRG1 (AAH18133) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human TCIRG1.
References
1. A specific subtype of osteoclasts secretes factors inducing nodule formation by osteoblasts. Henriksen K, Andreassen KV, Thudium CS, Gudmann KN, Moscatelli I, Cruger-Hansen CE, Schulz AS, Dziegiel MH, Richter J, Karsdal MA, Neutzsky-Wulff AV.Bone. 2012 Jun 19;51(3):353-361. 2. Impaired gastric acidification negatively affects calcium homeostasis and bone mass. Schinke T, Schilling AF, Baranowsky A, Seitz S, Marshall RP, Linn T, Blaeker M, Huebner AK, Schulz A, Simon R, Gebauer M, Priemel M, Kornak U, Perkovic S, Barvencik F, Beil FT, Del Fattore A, Frattini A, Streichert T, Pueschel K, Villa A, Debatin KM, RuegNat Med. 2009 Jun;15(6):674-81. 3. Differential Localization of Vacuolar H+-ATPases Containing a1, a2, a3, or a4 (ATP6V0A1-4) Subunit Isoforms Along the Nephron. Schulz N, Dave MH, Stehberger PA, Chau T, Wagner CA.Cell Physiol Biochem. 2007;20(1-4):109-20.
USBio References
No references available
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