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137711-HRP Mouse Anti-CD31 (Platelet Endothelial Cell Adhesion Molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1) (HRP)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P16284
Conjugate
HRP
Isotype
IgG1
Clone Number
158-2B3
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

Human CD31 is an adhesion molecule expressed on platelets, endothelial cells, leukocytes and their bone marrow precursors. CD31 plays a role in homophilic adhesion and heterophilic transendothelial migration.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Stimulated human leukocytes
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography from (low FBS containing) tissue culture supernatant.
Specificity
Recognizes human CD31. Reacts with domain 1 of CD31 and blocks homophilic interaction and heterophilic transendothelial migration (1).
References
1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 362-372, 2. P.J. Newman, (1997) J Clin Invest 99: 3-10., 3. K.L. Yong, et al, (1998) Blood 91: 1196-1205.
USBio References
No references available
Conjugates
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