Suitable for use in Flow Cytometry, Immunofluorescence, ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher.

Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin’s lymphoma.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Synthetic peptide corresponding to aa3-14 (Cys-QSNRELVVDFLS) from human Bcl-X.

Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.

Purified by Protein A/G affinity chromatography.

Recognizes human Bcl-X, ~27kD. Species Crossreactivity: mouse, rat and porcine.