POLR2J3, Recombinant, Human, aa1-115, His-Tag

Grade

Highly Purified

Shipping Temp

Blue Ice

Storage Temp

-20°C

DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Source

Recombinant protein corresponding to aa1-115 from human POLR2J3 at N-terminal, fused to His-tag expressed in E.coli.

Molecular Weight

~15.5kD (138aa)

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGS HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP MNAPPAF ESFLLFEGEK ITINKDTKVP NACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source

Recombinant, E. coli

Purity

~95% (SDS-PAGE)

Concentration

~0.5mg/ml

Form

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride,1mM DTT, 40% glycerol.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride,1mM DTT, 40% glycerol.

Purity

~95% (SDS-PAGE)

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138716 Anti-POLR2J3, Recombinant, Human, aa1-115, His-Tag (DNA-directed RNA Polymerase II Subunit RPB11-b2, POLR2J2, RPB11b1, RPB11b2)

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References

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