Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-200 Western Blot: 1:100-400 Immunocytochemistry: 1:100-500 Immunohistochemistry: 1:100-500 (frozen) Immunohistochemistry: 1:50-200 (paraffin sections) Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CAPN1 (Tyr387~Asp694); MGHHHHHHSGSEF- YPAT FWVNPQFKIR LEEVDDADDY DNRESGCSFL LALMQKHRRR ERRFGRDMET IGFAVYQVPR ELAGQPVHLK RDFFLANASR AQSEHFINLR EVSNRIRLPP GEYIVVPSTF EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE EIDDNFKTLF SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL NKKLHELIIT RYSEPDLAVD FDNFVCCLVR LETMFRFFKL LDTD
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse Calpain 1, Large Subunit (CAPN1)