Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.

Suitable for use in Sandwich FLISA and Western Blot. Other applications not tested.

Sandwich FLISA: 1-2ug/ml (assuming 100ul/well) will detect at least 1000pg/ml of recombinant Human IP-10 when used with affinity purified anti-human IP-10 (Biotin) as the detection antibody at a concentration of ~0.25ug/ml. Western Blot: 0.25-0.5ug/ml (non-reducing) or 1-2ug/ml (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant human IP-10 is 0.24ng/lane under non-reducing conditions and 3.91 under reducing conditions. Optimal dilutions to be determined by the researcher.

May be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 

Note: Applications are based on unconjugated antibody.

Recombinant protein corresponding to human IP-10 expressed in E. coli.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography

Recognizes human IP-10.