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145550 Sheep Anti-Nicalin (Ncl-1, NCLN, NET59, NICAstrin-Like protein)

Specifications
Clone Type
Polyclonal
Host
Sheep
Source
Human
Swiss Prot
Q969V3
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Nicalin (NICAstrin-Like proteIN) is a 60-62kD member of the nicastrin family of proteins. It is an ER-embedded transmembrane protein that is found in skeletal and cardiac muscle, hepatocytes and neurons. Nicalin interacts with 22kD TMEM147 and 130kD NOMO1 to form a 200-220kD complex that antagonizes Nodal signaling. Mature human nicalin is a 521 amino acid (aa) type I transmembrane glycoprotein. It contains a 480aa luminal/extracellular region (aa 43-522) plus a 20aa cytoplasmic domain (aa544-563). The luminal region possesses one likely inactive M28 Zn-peptidase-like domain (aa130-429). Four potential isoform variants are reported. One shows an alternative start site at Met75, a second contains a seven aa insertion after Gln543, a third contains a 102aa substitution for aa162-563, and a fourth shows a deletion of Gln446. Over aa1-522, human nicalin shares 95% aa sequence identity with mouse nicalin.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to aa1-522 from human Nicalin, S. frugiperda insect ovarian cell line Sf 21-derived UniProt # Q969V3).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Nicalin. Species Crossreactivity: mouse and rat
Conjugates
Pricing
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