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167465 Goat Anti-NSG1 (Brain Neuron Cytoplasmic Protein 1/2, Brain Specific mRNA b, Bsmrb, Neep21, Neuron specific gene family member 1, Neuron-specific Protein family member 1, PEP1)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Mouse
Isotype
IgG
Grade
Purified
Applications
E IC WB
Crossreactivity
Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Widely expressed in brain and spinal cord.

Applications
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:128,000 Western Blot: 0.1-0.3ug/ml Immunocytochemistry: 1-5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide with sequence EKGTKQPLLEDGFD-C, from the N-terminal domain of the protein sequence a(NP_077042.2). Species sequence homology: Human, bovine.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes mouse Nsg1 / Neep21. Species Crossreactivity: rat
References
1. Digilio L, Yap CC, Winckler B. Neurons in Rat Cultures: Preponderance of Single- and Double-Positive Cells, and Cell Type-Specific Expression of Neuron-Specific Gene Family Members, Nsg-1 (NEEP21) and Nsg-2 (P19). PLoS One. 2015 Oct 14; 10(10):e0140010. PMID: 26465886
USBio References
No references available
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