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168668 Mouse Anti-CD171 (NCAM-L1, CAML1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, S10, SPG1)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P32004 (Human)
Isotype
IgG1,k
Clone Number
UJ127
Grade
Affinity Purified
Applications
FC IF IHC IP WB
Crossreactivity
Hu
Gene ID
3897 (Human)
Shipping Temp
Blue Ice
Storage Temp
-20°C
(NCAM-L1, CAML1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, S10, SPG1)

Recognizes a cell surface protein of 220-240kDa, identified as L1 cell adhesion molecule. The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, and adducted thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, and neural migration as well as in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm. This MAb is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin e.g. neuroblastoma and Schwannomas. It does not bind to pediatric or adult brain.

Applications:  Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-1ug/million cells  Immunofluorescence: 1-2ug/ml  Immunoprecipitation: 1-2ug/500ug protein  Western Blot: 0.5-1.0ug/ml Immunohistochemistry: Frozen: 1-2ug/ml for 30 min at RT Optimal dilutions to be determined by the researcher.
Positive Control
Neuroblastomas or Schwannomas
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Homogenous suspension of 16 week human fetal brain. Cellular Localization: Cell surface
Form
Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 168668-AF.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human CD171 (NCAM-L1).
References
1. Kemshead J, et. al. International J Cancer, 1983; 31:187-195. 2. Patel, et. al. Biochem Soc Transactions, 1990; 18:274
USBio References
No references available
Conjugates
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