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168873-AF Mouse Anti-Thymidylate Synthase (dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase) (BSA & Azide Free)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
TMS715
Grade
Affinity Purified
Applications
E
Crossreactivity
Hu
Gene ID
7298 (Human)
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free
(dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase)

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Applications:  Suitable for use in Immunofluorescence, ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-1ug/million cells  Immunofluorescence: 0.5-1ug/ml  Western Blot: 0.25-0.5ug/ml  Immunoprecipitation: 0.5-1ug/500ug protein lysate Immunohistochemistry: Frozen & Formalin-fixed: 0.5-1ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher.
Positive Control
5-FU-resistant colon carcinoma cell lines: NCI H630R10, NCI H630R1; 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Colorectal, gastric, head & neck, and breast carcinomas.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant human thymidylate synthase. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168873.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human Thymidylate Synthase.
References
1. Johnston PG, et. al. Cancer Research, 1991, 51(24):6668-76. 2. Johnston PG, et. al. Cancer Research, 1992, 52(16):4306-12. 3. Johnston PG, et. al. Biochemical Pharmacology, 1993, 45(12):2483-6. 4. Johnston PG, et. al. Journal of Clinical Oncology, 1994, 12(12):2640-7. 5. Johnston PG, et. al. Cancer Research, 1995, 55(7):1407-12. 6. Johnston PG, et. al. Journal of the National Cancer Institute, 1997, 89(4):308-13. 7. Pestalozzi BC, et. al. Journal of Clinical Oncology, 1997, 15(5):1923-31.
USBio References
No references available
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